Long-term cryoconservation and stability of vitamin C in serum samples of the European prospective investigation into cancer and nutrition.
Author(s): Jenab M, Bingham S, Ferrari P, Friesen MD, Al-Delaimy WK, Luben R, Wareham N, Khaw KT, Riboli E
Publication: Cancer Epidemiol Biomarkers Prev, 2005, Vol. 14, Page 1837-40
PubMed ID: 16030126 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to assess the effects of storage on vitamin C levels in plasma.
Conclusion of Paper
A larger decline in vitamin C levels was observed in women than in men and in specimens with baseline vitamin C levels in the highest quintile. Patient smoking status had no effect on the rate of vitamin C loss in specimens. Baseline and final vitamin C values showed a moderate correlation in men and women, but the correlation was strongest in specimens with baseline values in the lowest quintile.
Studies
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Study Purpose
The purpose of this study was to assess the effects of storage on vitamin C levels in plasma. Prior to analysis all specimens were preserved with metaphosphoric acid and stored at -70 degrees C for less than 1 week.
Summary of Findings:
Vitamin C levels declined by 8.2% per year in men and 17.6% per year in women with baseline vitamin C values in the lowest quintile and by 24.6% per year in men and 24.2% per year in women with baseline vitamin C values in the highest quintile. Patient smoking status had no effect on the rate of vitamin C loss in specimens. Baseline and final vitamin C values showed a moderate correlation in men (r=0.57; p<0.00001) and women (r=0.52; p<0.0001), but the correlation was strongest in specimens with baseline values in the lowest quintile (r=0.62 and 0.72 for men and women respectively). In men, smokers had significantly lower baseline vitamin C levels then never-smokers.
Biospecimens
Preservative Types
- Frozen
- Other Preservative
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Fluorometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration < 1 week
7-11 years
Storage Storage temperature -70 degrees C
-196 degrees C
Preaquisition Patient gender Female
Male
Preaquisition Biomarker level Vitamin C level in lowest quintile
Vitamin C level in highest quintile
Preaquisition Other drugs Smoker
Never smoked