Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Determinants of DNA yield and quality from buccal cell samples collected with mouthwash.

Author(s): Feigelson HS, Rodriguez C, Robertson AS, Jacobs EJ, Calle EE, Reid YA, Thun MJ

Publication: Cancer Epidemiol Biomarkers Prev, 2001, Vol. 10, Page 1005-8

PubMed ID: 11535555 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to evaluate whether the yield of human DNA extracted from buccal specimens is influenced by the following aspects of buccal cell collection: (1) "swish" time, (2) specimen collection after tooth brushing, and (3) a delay in specimen processing.

   Summary of Findings:

   Swish time (30 vs. 60 s) did not affect the yield of extracted DNA. Tooth brushing up to one hour before sample collection reduced human DNA yield by 40%. While, human DNA yields did not significantly differ when processed within 2 to 5 days of collection, samples held for 10 and 30 days resulted in significantly less human DNA than samples held for two days.

   Biospecimens

   • Cell - Buccal
   • Bodily Fluid - Saliva

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Spectrophotometry
   DNA	Dot blot or slot blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Swish time (30 s) Swish time (60 s) Toothbrushing prior to collection
   Storage	Storage duration	2 d 5 d 10 d 30 d

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2. Study Purpose

   The purpose of this study was to evaluate if DNA integrity, as determined by successful PCR amplification of different sized beta-globin fragments, is influenced by the following aspects of buccal cell collection: (1) "swish" time, (2) specimen collection after tooth brushing, and (3) a delay in specimen processing.

   Summary of Findings:

   DNA integrity, as measured by the successful PCR amplification of three size beta-globin fragments, was unaffected by swish time or tooth brushing an hour before collection, while PCR failure rates for specimens stored for 5 days or longer ranged from 8 to 18%.

   Biospecimens

   • Cell - Buccal
   • Bodily Fluid - Saliva

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Swish time (30 s) Swish time (60 s) Toothbrushing prior to collection
   Storage	Storage duration	2 d 5 d 10 d 30 d
   PCR Specific	Length of gene fragment	268 bp 536 bp 989 bp

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