A simple mouthwash method for obtaining genomic DNA in molecular epidemiological studies.
Author(s): Lum A, Le Marchand L
Publication: Cancer Epidemiol Biomarkers Prev, 1998, Vol. 7, Page 719-24
PubMed ID: 9718225 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to determine the effects of storage of mouthwash specimens on DNA yield and genotyping success.
Summary of Findings:
Storage of mouthwash specimens at room temperature or 37 degrees C for 1 week or -20 degrees C for up to 6 months had no effects on DNA yield or on PCR RFLP genotyping success. The authors report that all 64 specimens yielded high molecular weight DNA, and four genes (GSTM1, GSTT1, CYP1A1, and CYP2E1) were successfully genotyped for all specimens, regardless of patient age, gender, race, or smoking status.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform DNA Electrophoresis DNA Restriction fragment length polymorphism DNA SNP assay DNA Spectrophotometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature -20 degrees C
Room temperature
37 degrees C
Storage Storage duration 0 days
1 week
3 months
6 months
SNP assay Specific Targeted nucleic acid GSTM1
GSTT1
CYP1A1
CYP2E1