Stability of vitamins A, C, and E, carotenoids, lipids, and testosterone in whole blood stored at 4 degrees C for 6 and 24 hours before separation of serum and plasma.
Author(s): Key T, Oakes S, Davey G, Moore J, Edmond LM, McLoone UJ, Thurnham DI
Publication: Cancer Epidemiol Biomarkers Prev, 1996, Vol. 5, Page 811-4
PubMed ID: 8896892 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to assess the stability of circulating levels of vitamins A, C, E, as well as carotenoids, lipids, and steroids in plasma and serum after a delay at 4 degrees C prior to separation.
Summary of Findings:
A 24 hour delay at 4 degrees prior to blood separation resulted in a significant decrease in retinol, alpha-carotene, and vitamin C levels in plasma, and an increase in serum testosterone; the magnitude of the observed changes ranged between 3 and 6% in relation to 2 hour controls.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Small molecule HPLC Small molecule Fluorometry Steroid Radioimmunoassay Steroid Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 2 h
6 h
24 h
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated