Measurement of organochlorine levels in postprandial serum or in blood collected in serum separator tubes.

Author(s): Longnecker MP, Bernstein L, Bird CL, Yancey AK, Peterson JC

Publication: Cancer Epidemiol Biomarkers Prev, 1996, Vol. 5, Page 753-5

PubMed ID: 8877068 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collection and storage container and fasting on serum PCB and DDE levels. Specimens were frozen at -20 or -70 degrees C until analysis.

   Summary of Findings:

   A strong correlation was observed between fasting lipid adjusted PCB and DDE collected in plain tubes and those collected in SST or after eating. PCB and DDE levels were lower when fasting blood was collected and stored in SST rather than plain tubes for 5 days, but not 1 day. The reduction was greater for high molecular weight PCBs than low molecular weight PCBs. After eating, serum lipids increased, and lipid adjusted levels of DDE decreased slightly.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Lipid	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer
   Small molecule	GC- flame ionization

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Type of storage container	Vacutainer tube Serum separator tube
   Storage	Storage duration	1 day 5 days
   Storage	Storage temperature	2-8 degrees C
   Preaquisition	Patient diet	Fasting After eating

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