Validity for epidemiological studies of long-term cryoconservation of steroid and protein hormones in serum and plasma.

Author(s): Bolelli G, Muti P, Micheli A, Sciajno R, Franceschetti F, Krogh V, Pisani P, Berrino F

Publication: Cancer Epidemiol Biomarkers Prev, 1995, Vol. 4, Page 509-13

PubMed ID: 7549807 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to assess the stability of estradiol, testosterone, progesterone, and prolactin hormones in serum after long term frozen storage.

   Summary of Findings:

   Estradiol, total testosterone, and prolactin did not differ significantly among serum specimens stored for 6 months versus 3 years, whereas free testosterone increased over time by as much as 30%. Serum progesterone concentrations decreased as a function of time by up to 40% after 3 years.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Steroid	Radioimmunoassay
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	6 months 1 yr 1.5 yr 2 yr 3 yr
   Storage	Storage temperature	-80 degrees C

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2. Study Purpose

   The purpose of this study was to assess the stability of estradiol, testosterone, progesterone, and prolactin hormones in plasma after long term frozen storage.

   Summary of Findings:

   Plasma estradiol, total testosterone, and progesterone concentrations varied as a function of frozen storage. Plasma estradiol and progesterone concentrations decreased progressively with storage duration. Variability of plasma estradiol and total testosterone levels were greater in specimens stored for 1 year or more. Conversely, plasma prolactin levels remained stable for up to 3 years.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Steroid	Radioimmunoassay
   Protein	Radioimmunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	-80 degrees C
   Storage	Storage duration	6 months 1 yr 2 yr 3 yr

   View more