Loss of antigenicity in stored sections of breast cancer tissue microarrays

Author(s): Fergenbaum J, Garcia-Closas M, Hewitt SM, Lissowska J, Sakoda L, Sherman ME

Publication: Cancer Epidemiol Biomarkers and Prev, 2004, Vol. 13, Page 667

PubMed ID: 15066936 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   To determine if immunostaining of ERalpha, PR, and HER-2 is affected by storage of FFPE tissue sections for 6 months compared to those freshly sectioned.

   Summary of Findings:

   The percentage of positive cases among sections stored for 6 months and those freshly cut were equivalent for ERalpha (59%), but significantly reduced for PR (56 vs 64%) and HER-2 (45 vs 64%). Further, the percentage of immunopositive cells per case was significantly lower in stored sections versus freshly cut sections for ERalpha (27 vs 38%), PR (30 vs 70%), and HER-2 (0 vs 30%).

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Tissue microarray
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	< 1 day 6 months
   Immunohistochemistry Specific	Targeted peptide/protein	HER-2 ER alpha PR

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