NIH, National Cancer Institute, Division of Cancer Treatment and Diagnosis (DCTD) NIH - National Institutes of Health National Cancer Institute DCTD - Division of Cancer Treatment and Diagnosis
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Do the histologic features and results of breast cancer biomarker studies differ between core biopsy and surgical excision specimens?

Author(s): Burge CN, Chang HR, Apple SK

Publication: Breast, 2006, Vol. 15, Page 167-72

PubMed ID: 16095904 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to evaluate differences in diagnosis of tumor type and grade as well as differences in biomarker analysis between core needle biopsies and subsequent surgical excisions of breast tumor tissue.

Conclusion of Paper

Overall, relatively high concordance rates were obtained for all histological and biomarker studies comparing core needle biopsies with surgical excision specimens, including 100% concordance for histological type of cancer. Furthermore, immunohistochemistry (IHC) and fluorescence in situ hybridization (FISH) results agreed with respect to HER2 status 94% of the time.

Studies

  1. Study Purpose

    The purpose of this study was to determine if analysis of histological features and prognostic markers yields different outcomes for core needle biopsies and surgical excision specimens, including both lumpectomies and mastectomies. 87 different breast cancer cases were included in the study.

    Summary of Findings:

    The histological type of cancer determined from a core biopsy was the same as that determined from a surgical excision specimen 100% of the time. The concordance rates of modified Bloom and Richardson grades between excisional and core biopsies were 81%, 83% and 65% for low, intermediate and high grade tumors. Of the 20 discordent specimens, the grade was higher in the excisional specimen in 17 cases. The concordance rate for ER was 95% with 2 cases positive by core biopsy and negative by excisional biopsy and two cases positive by excisional biopsy and negative by core biopsy. PR and p53 concordance rates were 89% and 86% respectively, each having an additional 5 cases positive by excisional biopsy and 5 by core biopsy. HER2 status using IHC had a concordance rate of 96% with all 3 discordent specimens being positive by excisional biopsy but negative by core biopsy. The concordance rate of HER2 status by FISH analysis was 100%, but FISH results were only available from both specimen types in 18 out of 87 cases. In the 78 out of 87 excision specimens which had results from FISH analysis, IHC and FISH results agreed, with respect to HER2 status, 94% of the time.

    Biospecimens
    Preservative Types
    • Formalin
    Diagnoses:
    • Neoplastic - Carcinoma
    Platform:
    AnalyteTechnology Platform
    Protein Immunohistochemistry
    DNA FISH
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Immunohistochemistry Specific Targeted peptide/protein ER
    PR
    HER2
    p53
    FISH Specific Targeted nucleic acid HER2
    FISH Specific Technology platform FISH
    Immunohistochemistry
    Biospecimen Acquisition Method of tissue acquisition Core needle biopsy
    Surgical resection
    View more

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