Optimal tumor sampling for immunostaining of biomarkers in breast carcinoma.
Author(s): Tolles J, Bai Y, Baquero M, Harris LN, Rimm DL, Molinaro AM
Publication: Breast Cancer Res, 2011, Vol. 13, Page R51
PubMed ID: 21592345 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of the number of 20x field images analyzed using AQUA on the quantification of immunofluorescence for cancer biomarkers including estrogen receptor (ER), human epidermal growth factor receptor 2 (HER2), serine/threonine protein kinase (AKT), extracellular-signal-regulated kinase (ERK), ribosomal protein S6 kinase beta-1 (S6K1), glyceraldehyde 3-phosphate dehydrogenase (GAPDH), cytokeratin, and microtubule-associated protein (MAP)-Tau. Due to the small sample size and number of fields of view, a mathematical simulation was performed for all biomarkers except MAP-Tau, for which direct sampling of 122 whole section slides and the simulation were performed. Only ER-positive breast cancer cases were included.
Summary of Findings:
The optimum number of 20x field images required to estimate the expression of a biomarker in a whole tissue section, as assessed by AQUA score, varied based on biomarker and ranged from 3 (cytokeratin) to 14 (MAP-Tau, simulated and direct sampling). In general, a higher number of 20x fields of view were required for markers showing more heterogeneity.
Biospecimens
Preservative Types
- Formalin
Diagnoses:
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Protein Immunohistochemistry Morphology Fluorescent microscopy Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Aliquot size/volume 1-35 20x fields of view
Immunohistochemistry Specific Targeted peptide/protein ER
HER2
AKT
ERK
S6K1
GAPDH
Cytokeratin
MAP-Tau