Epidermal growth factor receptor in breast cancer: storage conditions affecting measurement, and relationship to steroid receptors.

Author(s): McLeay WR, Horsfall DJ, Seshadri R, Morrison DA, Saccone GT

Publication: Breast Cancer Res Treat, 1992, Vol. 22, Page 141-51

PubMed ID: 1391979 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study determined whether the duration and temperature of frozen storage alters quantifiable epidermal growth factor receptor (EGF-R) activity in breast cancer tissue specimens.

   Summary of Findings:

   Quantifiable EGF-R activity in breast cancer specimens was reduced by approximately 75% after freezing irrespective of temperature or duration. Subsequent freezing of broken cell pellets prepared from frozen tissue specimens resulted in a total loss of EGF-R activity in the majority of specimens.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Frozen

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Receptor binding

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	Liquid nitrogen -20 degrees C -70 degrees C
   Storage	Storage duration	2 d 5 d 7 d 14 d 21 d

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