Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

Tissue pH as an indicator of mRNA preservation in human post-mortem brain.

Author(s): Kingsbury AE, Foster OJ, Nisbet AP, Cairns N, Bray L, Eve DJ, Lees AJ, Marsden CD

Publication: Brain Res Mol Brain Res, 1995, Vol. 28, Page 311-8

PubMed ID: 7723629 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This paper examined the potential relationship between mRNA degradation and postmortem tissue pH in neurodegenerative and normal brain specimens; the variables of age, pathology, and rapidity of death were also investigated.

   Summary of Findings:

   There were no correlations among any of the following variables: tissue pH, age, pathology, postmortem interval, or rapidity of death. In situ hybridization histochemistry for beta-tubulin and aldolase C expression revealed a stronger signal in specimens with a pH above 6.5, with a significant correlation between probe binding and tissue pH observed. This correlative relationship among RNA degradation and tissue pH was supported by electrophoretic and Northern blot analysis. There was not a significant correlation among probe binding and postmortem interval.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Parkinson's Disease
   • Normal

   Platform:

   Analyte	Technology Platform
   RNA	In situ hybridization
   RNA	Electrophoresis
   RNA	Northern blot

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Postmortem interval	5-51 h
   Preaquisition	Rapidity of death	Slow death Rapid death
   Preaquisition	Patient age	40-91 y
   Biospecimen Aliquots and Components	pH	5 6

   View more