Effect of pre- and postmortem variables on specific mRNA levels in human brain.

Author(s): Burke W J, O'Malley K L, Chung H D, Harmon S K, Miller J P, Berg L

Publication: Brain Res Mol Brain Res, 1991, Vol. 11, Page 37

PubMed ID: 1662743 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   Polymerase chain reaction (PCR) was used to investigate which of eight pre- and four postmortem factors affect the stability of PNMT, three splice forms of APP (APP695, APP751, and APP770) and beta-actin mRNAs in the C1 region in the rostral ventral lateral medulla of human autopsy brain.

   Summary of Findings:

   The authors report that of the eight premortem variables examined, hypoxia was associated with decreased levels of PNMT and seizure activity was associated with increased levels of APP751. Of the four postmortem variables examined, postmortem interval and storage interval correlated with depressed levels of APP751 and beta-actin. The death refrigeration interval correlated with increased levels of total APP and APP695.

   Biospecimens

   • Tissue - Brain

   Preservative Types

   • Frozen

   Diagnoses:

   • Autopsy
   • Not specified

   Platform:

   Analyte	Technology Platform
   DNA	Southern blot
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Patient age	32 y 34 y 37 y 43 y 57 y 61 y 63 y 64 y 71 y 72 y
   Preaquisition	Diagnosis/ patient condition	Fever No fever Systemic infection No systemic infection Seizure activity No seizure activity Hypoxia No hypoxia 0 hours in coma 0.1 hours in coma 0.2 hours in coma 0.3 hours in coma 0.5 hours in coma 0.8 hours in coma 2.0 hours in coma 2.3 hours in coma 6.8 hours in coma 30.6 hours in coma On a respirator Not on a respirator
   Preaquisition	Diagnosis/ patient condition	Systemic infection No systemic infection
   Preaquisition	Blood pressure	Hypotension No hypotension
   Preaquisition	Diagnosis/ patient condition	On a respirator Not on a respirator
   Preaquisition	Diagnosis/ patient condition	Seizure activity No seizure activity
   Preaquisition	Diagnosis/ patient condition	Hypoxia No hypoxia 0 hours in coma 0.1 hours in coma 0.2 hours in coma 0.3 hours in coma 0.5 hours in coma 0.8 hours in coma 2.0 hours in coma 2.3 hours in coma 6.8 hours in coma 30.6 hours in coma
   Preaquisition	Postmortem interval	2.9 h 4.2 h 4.3 h 7.0 h 8.4 h 14.3 h 17.3 h 17.6 h 20 h 24.7 h 3.1 h PMI + coma 7.0 h PMI + coma 8.7 h PMI + coma 11.0 h PMI + coma 14.8 h PMI + coma 18.4 h PMI + coma 19.6 h PMI + coma 20.1 h PMI + coma 24.7 h PMI + coma 34.9 h PMI + coma
   Storage	Storage duration	8 days 23 days 24 days 32 days 221 days 235 days 396 days 500 days 1221 days 1229 days
   Preaquisition	Postmortem interval	3.1 h PMI + coma 7.0 h PMI + coma 8.7 h PMI + coma 11.0 h PMI + coma 14.8 h PMI + coma 18.4 h PMI + coma 19.6 h PMI + coma 20.1 h PMI + coma 24.7 h PMI + coma 34.9 h PMI + coma
   Preaquisition	Postmortem condition(s)	0-4 h before refrigeration 1-22 h refrigerated

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