Blood gas analysis: effect of air bubbles in syringe and delay in estimation.
Author(s): Biswas CK, Ramos JM, Agroyannis B, Kerr DN
Publication: Br Med J (Clin Res Ed), 1982, Vol. 285, Page 1659-60
PubMed ID: 6802352 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of blood storage temperature and duration on the analysis of venous and arterial blood gas.
Summary of Findings:
The P02 was unaffected by storage of venous blood at 0 degrees C for up to 30 min prior to analysis, but it was reduced in venous blood stored for 60 or 120 min at 0 degrees C compared to venous blood analyzed within 5 min. The pCO2, and bicarbonate increased and pH decreased when venous blood was stored for 120 min at 0 degrees C compared to venous blood analyzed after 5 min, but no significant effects were observed after shorter delays in processing. The PO2 was reduced when venous blood was stored for 20 or 30 min at 4 or 22 degrees C compared to blood analyzed after 5 min, but the PCO2 was unaffected by storage at 4 or 22 degrees C for up to 30 min. When arterial blood was stored at 22 or 0 degrees C, the PO2 decreased within 10 and 60 min, respectively, compared to levels in arterial blood analyzed after 5 min. The authors note the decrease in P02 occurred faster in arterial blood than venous blood most likely due to the higher initial levels.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
- Other diagnoses
Platform:
Analyte Technology Platform Gas Clinical chemistry/auto analyzer Small molecule pH Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage temperature 0 degrees C (ice)
4 degrees C
22 degrees C
Storage Storage duration 5 min
10 min
20 min
30 min
60 min
120 min
Preaquisition Diagnosis/ patient condition Renal failure
Biospecimen Acquisition Anatomical location of blood draw Artery
Vein
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Study Purpose
The purpose of this study was to determine the effects of delayed processing of syringes containing air bubbles on the analysis of blood gas.
Summary of Findings:
When 0.1 ml-0.5 ml of air was added to 1 mL blood, the PO2 was significantly increased after 2 min and the PCO2 was decreased after 3 min compared to levels measured upon immediate analysis. A similar increase in PO2 occurred when the 0.1 ml air was present as froth in 2 mL of blood rather than as a single bubble.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Gas Clinical chemistry/auto analyzer Small molecule pH Small molecule Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Biospecimen components 0.1 ml air bubble in 1 mL blood
0.2 mL air bubble in 1 mL blood
0.5 mL air bubble in 1 mL blood
0.1 mL air froth in 2 mL blood
Storage Time at room temperature 0 min
1 min
2 min
3 min
4 min
5 min