Blood gas analysis: effect of air bubbles in syringe and delay in estimation.

Author(s): Biswas CK, Ramos JM, Agroyannis B, Kerr DN

Publication: Br Med J (Clin Res Ed), 1982, Vol. 285, Page 1659-60

PubMed ID: 6802352 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood storage temperature and duration on the analysis of venous and arterial blood gas.

   Summary of Findings:

   The P02 was unaffected by storage of venous blood at 0 degrees C for up to 30 min prior to analysis, but it was reduced in venous blood stored for 60 or 120 min at 0 degrees C compared to venous blood analyzed within 5 min. The pCO2, and bicarbonate increased and pH decreased when venous blood was stored for 120 min at 0 degrees C compared to venous blood analyzed after 5 min, but no significant effects were observed after shorter delays in processing. The PO2 was reduced when venous blood was stored for 20 or 30 min at 4 or 22 degrees C compared to blood analyzed after 5 min, but the PCO2 was unaffected by storage at 4 or 22 degrees C for up to 30 min. When arterial blood was stored at 22 or 0 degrees C, the PO2 decreased within 10 and 60 min, respectively, compared to levels in arterial blood analyzed after 5 min. The authors note the decrease in P02 occurred faster in arterial blood than venous blood most likely due to the higher initial levels.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	0 degrees C (ice) 4 degrees C 22 degrees C
   Storage	Storage duration	5 min 10 min 20 min 30 min 60 min 120 min
   Preaquisition	Diagnosis/ patient condition	Renal failure
   Biospecimen Acquisition	Anatomical location of blood draw	Artery Vein

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2. Study Purpose

   The purpose of this study was to determine the effects of delayed processing of syringes containing air bubbles on the analysis of blood gas.

   Summary of Findings:

   When 0.1 ml-0.5 ml of air was added to 1 mL blood, the PO2 was significantly increased after 2 min and the PCO2 was decreased after 3 min compared to levels measured upon immediate analysis. A similar increase in PO2 occurred when the 0.1 ml air was present as froth in 2 mL of blood rather than as a single bubble.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Gas	Clinical chemistry/auto analyzer
   Small molecule	pH
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen components	0.1 ml air bubble in 1 mL blood 0.2 mL air bubble in 1 mL blood 0.5 mL air bubble in 1 mL blood 0.1 mL air froth in 2 mL blood
   Storage	Time at room temperature	0 min 1 min 2 min 3 min 4 min 5 min

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