Using a commercially available DNA extraction kit to obtain high quality human genomic DNA suitable for PCR and genotyping from 11-year-old saliva saturated cotton spit wads.
Author(s): Ehli EA, Lengyel-Nelson T, Hudziak JJ, Davies GE
Publication: BMC Res Notes, 2008, Vol. 1, Page 133
PubMed ID: 19102763 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effect of storage of saliva at -20 degrees C for 11 years on DNA integrity.
Conclusion of Paper
Long term frozen storage of saliva saturated cotton wads did not inhibit the ability to extract DNA from buccal cells suitable for PCR-based genotyping.
Studies
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Study Purpose
The purpose of this study was to determine the effect of storage of saliva at -20 degrees C for 11 years on DNA integrity.
Summary of Findings:
Long term frozen storage of saliva saturated cotton wads did not inhibit the ability to extract DNA from buccal cells suitable for PCR-based genotyping. DNA yields, as determined by spectrophotometer, were variable and seemed to correlate with the amount of saliva contained in the spit wad. Average yield was 17.3 +/- 11.9 ug. Average quality as measured by A260/A280 ratio was 1.937 +/- 0.226 prior to concentration. PCR amplification of a functional polymorphism in the serotonin transporter gene promoter region was successful for 98.3% of the 783 samples.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
- Other diagnoses
Platform:
Analyte Technology Platform DNA Spectrophotometry DNA PCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition ADHD
Biospecimen Aliquots and Components Aliquot size/volume Various amounts of saliva