Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Stable hepatitis C virus RNA detection by RT-PCR during four days storage.

Author(s): de Moreau de Gerbehaye AI, Bodéus M, Robert A, Horsmans Y, Goubau P

Publication: BMC Infect Dis, 2002, Vol. 2, Page 22

PubMed ID: 12366870 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to determine the effects of storage for up to 4 days at room temperature or 4 degrees C of serum or clotted blood specimens on hepatitis c virus (HCV) detection by RT-PCR. After the allotted storage duration, all aliquots were kept at -80 degrees C until analysis.

   Summary of Findings:

   Over the 4 days of storage at room temperature or 4 degrees C the average levels of HCV RNA detected remained within 30% of initial values. However, in one serum specimen stored at room temperature for 24 h, a very low level of HCV RNA was detected. No differences in HCV levels were identified when specimens were stored as clotted blood versus serum or when serum centrifugation was delayed by 6 h rather than centrifuged within 1 h.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Hepatitis

   Platform:

   Analyte	Technology Platform
   RNA	RT-PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 h 6 h 24 h 48 h 72 h 96 h
   Storage	Storage temperature	Room temperature 4 degrees C
   Biospecimen Aliquots and Components	Blood and blood products	Serum Whole blood
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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