The chemical composition of normal human red blood cells, including variability among centrifuged cells.

Author(s): Keitel HG, BERMAN H, JONES H, MACLACHLAN E

Publication: Blood, 1955, Vol. 10, Page 370-6

PubMed ID: 14363319 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of patient gender and sampling position within the red blood cell pack on electrolyte levels and water content. Red cells were obtained by centrifugation of heparinated whole blood and removal of the buffy coat and plasma.

   Summary of Findings:

   Cells from lower in the red cell pack contained more hemoglobin and less reticulocytes, water, potassium, sodium, chloride and phosphorous compared to cells obtained from higher in the cell pack, but plasma contamination was similar between sampling levels. The plasma and red blood cells from male patients had more sodium than specimens from females, but no other gender differences were observed. The authors conclude that the entire red cell pack, as obtained with a constricted centrifuge tube, should be used for analysis.

   Biospecimens

   • Cell - Red Blood Cells
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Flame emission photometry
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen components	Top 6% of cell pack Middle of cell pack Bottom 6% of cell pack
   Preaquisition	Patient gender	Female Male
   Biospecimen Aliquots and Components	Blood and blood products	Multiple sampling positions examined Red blood cells

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