Oxidative injury as contributory factor for red cells storage lesion during twenty eight days of storage.

Author(s): Chaudhary R, Katharia R

Publication: Blood Transfus, 2012, Vol. 10, Page 59-62

PubMed ID: 22044961 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of storage duration at 4 degrees C on markers of red cell damage in buffy coat depleted red blood cell units in SAGM. Aliquots were removed aseptically at each time-point.

   Summary of Findings:

   Plasma Hb, plasma potassium, MDA, Hb oxidation and LDH increased progressively over the 28 days of storage (p<0.001, all). Osmotic lysis increased from 1.07% to 16.2% (p<0.005) during storage for 28 days at 4 degrees C. At day 14 and 28, but not day 0 there was a modest correlation between lipid peroxidation and Hb (r=0.578, p<0.001, and r=0.513, p<0.005, respectively). There was a modest correlation between plasma hemoglobin and the conversion of hemoglobin to methemaglobin (r=0.567).

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Protein	Hematology/ auto analyzer
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage duration	0 days 14 days 28 days

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