Storage of buffy-coat-derived platelets in additive solution: in vitro effects on platelets of the air bubbles and foam included in the final unit.

Author(s): Sandgren P, Saeed K

Publication: Blood Transfus, 2011, Vol. 9, Page 182-8

PubMed ID: 21084001 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of air bubbles during room temperature storage of plts on plt quality. Buffy coats with plasma were prepared from citrate-phosphate-dextrose (CPD)/saline-adenine-glucose-mannitol (SAG-M) blood and stored overnight before isolation of plts. After isolation air bubbles and foam were excluded from one of each sample pairs while the other was stored with the air bubbles and foam. Plts were stored at room temperature on a flat bed agitator and sampled aseptically.

   Summary of Findings:

   While plt counts and MPV were stable in plts stored without air bubbles, plt counts and MPV decreased progressively in plts stored with air bubbles. Compared to plts stored for 2 days, a slight increase was noted in LDH levels after 7 days of room temperature storage without air bubbles, but a much larger increase was observed after 5 or 7 days of room temperature storage with air bubbles. pH was unaffected by room temperature storage of plts, with or without air bubbles. pCO2, bicarbonate and ATP levels decreased with room temperature storage of plts and were lower on day 7 in specimens stored with air bubbles than those stored without. The PO2 increased during room temperature storage of specimens without air bubbles and the PO2 was significantly higher in specimens stored with air bubbles than those without, but PO2 decreased between day 2 and 5 in specimens with air bubbles. HSR and ESC decreased during room temperature storage of plts and were lower in specimens stored with air bubbles than those stored without air bubbles. The percentage of CD62P expressing plts increased and CD42b expressing plts decreased during storage, and significantly fewer CD42b expressing plts and significantly more CD62P expressing plts were observed by day 5 and day 7, respectively, in specimens stored with air bubbles than those stored without. The percentages of CD41, CD61 and GPIIb/IIIa expressing platelets were unaffected by room temperature storage, with or without air bubbles. The authors report that swirling effect remained at acceptable levels throughout room temperature storage of plts.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Cell count/volume	Flow cytometry
   Protein	Spectrophotometry
   Morphology	Macroscopic observation
   Morphology	Hematology/ auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Small molecule	Bioassay
   Gas	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	2 days 5 days 7 days
   Storage	Storage conditions	With air bubbles Without air bubbles

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