Influence of centrifuge brake on residual platelet count and routine coagulation tests in citrated plasma.

Author(s): Daves M, Giacomuzzi K, Tagnin E, Jani E, Adcock Funk DM, Favaloro EJ, Lippi G

Publication: Blood Coagul Fibrinolysis, 2014, Vol. 25, Page 292-5

PubMed ID: 24389587 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of centrifugation and centrifuge braking on Plt counts and coagulation test results in plasma. Sodium citrate anticoagulated platelet poor plasma (PPP) specimens were collected from 25 patients in the oral anticoagulant clinic and 25 patients in the outpatient clinic sent for routine blood testing. Specimens were then transferred to the laboratory for Plt counts. Specimens were centrifuged at 1500g for 15 min at room temperature with the brake set to on or off.

   Summary of Findings:

   The Plt counts decreased by 96.9% and 97.5% after centrifugation, with the brake set to on or off, respectively. When the centrifuge brake was used, the Plt counts and fibrinogen levels were higher (p=0.009 and p<0.001, respectively), and the PT was longer (p<0.001) than when the centrifuge brake was set to off, but the APTT was not affected by braking. Very strong correlation for APTT (r=1.00, p<0.001) and PT (r=1.00, p<0.001), and strong correlations in fibrinogen levels (r=0.89, p<0.001) were observed between specimens centrifuged with the brake set to on and those with it set to off, but no significant correlations between Plt counts were observed. A slight but significant increase in the number of specimens having a having a higher Plt count than allowed by the Clinical Laboratory Standards Institute was observed when specimens were centrifuged with the brake on (14% versus 8%, p<0.01). The authors report similar results in specimens from outpatients and those on oral anticoagulants.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Other diagnoses

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Flow cytometry
   Morphology	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	Oral anticoagulant therapy patient Outpatient
   Biospecimen Aliquots and Components	Centrifugation	With braking Without braking Not centrifuged
   Preaquisition	Other drugs	On oral anticoagulant therapy No oral anticoagulant therapy

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