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Sodium citrate vacuum tubes validation: preventing preanalytical variability in routine coagulation testing.

Author(s): Lima-Oliveira G, Lippi G, Salvagno GL, Montagnana M, Picheth G, Guidi GC

Publication: Blood Coagul Fibrinolysis, 2013, Vol. 24, Page 252-5

PubMed ID: 23160241 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of vacuum tube brand on APTT, PT, and fibrinogen measurements in sodium citrate plasma. Plasma was frozen at -70 degrees C until analysis.

   Summary of Findings:

   APTT measurements were statistically and clinically significantly different between specimens collected in the Venosafe tubes and the Vacuette tubes and between specimens collected in the Labor Import tubes and the Venosafe, Vacuette, Vacutainer, and Monovette tubes. Specimens collected in the Vacuette tubes yielded the shortest average APTT while specimens collected in the Labor Import tubes yielded the longest average APTT. Statistically significant but clinically insignificant differences in APTT measurements were also seen between specimens collected in the Venosafe and Vacutainer tubes. PT measurements were statistically and clinically significantly different between specimens collected in the Venosafe tubes and the Vacuette, Vacutainer, and Monovette tubes, between specimens collected in the Vacuette tubes and the Labor Import and Monovette tubes, and between specimens collected in the Labor Import tubes and the Vacutainer and Monovette tubes. Specimens collected in Venosafe and Labor Import tubes yielded the longest average PT, while specimens in the Vacuette tubes yielded the shortest average PT. Statistically significant but clinically insignificant differences in PT measurements were also seen between specimens collected in the Vacutainer tubes and the Vacuette and Monovette tubes. Fibrinogen measurements were not significantly different between specimens collected in any of the tube brands.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Type of collection container/solution	Venosafe Vacuette BD Vacutainer Labor Import S-Monovette

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