Sample collection and platelet function testing: influence of vacuum or aspiration principle on PFA-100 test results.
Author(s): Lippi G, Ippolito L, Zobbi V, Sandei F, Favaloro EJ
Publication: Blood Coagul Fibrinolysis, 2013, Vol. 24(6), Page 666-9
PubMed ID: 23470650 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of manual versus vacuum aspiration of blood, collection tube type, and platelet activation solution on closure time using the PFA-100.
Conclusion of Paper
Use of manual aspiration during collection rather than vacuum aspiration resulted in shorter PFA-100 closure times. There were no significant differences between specimens collected by vacuum aspiration into Vacutainer tubes versus Monovettes.
Studies
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Study Purpose
The purpose of this study was to determine the effects of manual versus vacuum aspiration of blood, collection tube type, and platelet activation solution on closure time using the PFA-100. A Vacutainer tube was filled using vacuum aspiration with venipuncture of the right arm, followed by a Monovette tube filled by vacuum aspiration from a second venipuncture on the opposite arm, and lastly, another Monovette tube was filled by manual aspiration from the same second venipuncture site. All specimens were collected into sodium citrate with a tourniquet times of <30 sec.
Summary of Findings:
The closure times obtained when blood was collected by manual aspiration into Monovette tubes (SD-A) were significantly shorter than those obtained when blood was collected by vacuum aspiration into either Vacutainer (BD-V) or Monovette tubes (SD-V), regardless of which activation solution was used in the assay. SD-A closure time had a mean bias of -12 s when either collagen and ADP or collagen and epinephrine were used compared to results for SD-V specimens. SD-A closure times had mean biases of -9 s and -14 s when collagen and ADP or collagen and epinephrine were used, respectively, compared to results for BD-V specimens. The differences between SD-V and BD-V specimen closure times were not significant for either activation solution.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Cell count/volume Hematology/ auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Type of collection container/solution BD Vacutainer
Sarstedt S-Monovette
Biospecimen Acquisition Method of fluid acquisition Syringe draw
Vacuum tube
Hematology/ auto analyzer Specific Reaction solution Collagen and ADP
Collagen and epinephrine
Biospecimen Acquisition Anatomical location of blood draw Arm
Left and right sides compared