Influence of mechanical hemolysis of blood on two D-dimer immunoassays.

Author(s): Lippi G, Avanzini P, Zobbi V, Ippolito L

Publication: Blood Coagul Fibrinolysis, 2012, Vol. 23, Page 461-3

PubMed ID: 22576291 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of mechanically induced hemolysis and assay type on D-dimer measurement in plasma. Hemolysis was induced in aliquots A, B, and C by aspiration of whole blood 1, 2, or 3 times, respectively, through a 30 gauge needle. Hemolysis was not induced in a 4th aliquot. 4 pools of blood were used for analysis including a range of D-dimer concentrations.

   Summary of Findings:

   As expected, increasing the number of passages of whole blood through the fine needle induced higher levels of hemolysis. The concentrations of D-dimer in plasma decreased as the concentration of cell free hemoglobin increased, regardless of the type of immunoassay. The decreases in plasma D-dimer concentrations between the non-hemolyzed aliquots and hemolyzed aliquot A were not significant when measured by the HemosIL AcuStar D-dimer immunoassay, but they were significant when measured by the HemosIL D-dimer HS for ACL TOP immunoassay. All other decreases between the non-hemolyzed aliquots and hemolyzed aliquots B and C were significant for both immunoassays. The HemosIL AcuStar D-dimer immunoassay measured significantly higher concentrations of D-dimer in all specimens compared to the HemosIL D-dimer HS for ACL TOP immunoassay.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry
   Peptide	Immunoassay

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Hemolysis	Fine needle aspiration-induced Not induced
   Preaquisition	Biomarker level	Low D-dimer concentration Medium D-dimer concentration High D-dimer concentration Very high D-dimer concentration
   Immunoassay Specific	Technology platform	HemosIL AcuStar D-dimer HemosIL D-dimer HS for ACL TOP

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