Carbon monoxide releasing molecule-2 attenuates the anticoagulant and amplifies the hypofibrinolytic effects of hypothermia in human plasma in vitro.

Author(s): Nielsen VG, George SJ

Publication: Blood Coagul Fibrinolysis, 2011, Vol. 22, Page 67-72

PubMed ID: 21157299 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of CORM-2 exposure and incubating platelet poor plasma at temperatures below 37 degrees C on coagulation and fibrinolysis. Sodium citrate was used as an anticoagulant.

   Summary of Findings:

   Exposure of plasma to hypothermic temperatures resulted in prolonged onset and slower velocity of clot growth, however, clot strength was unaffected. The onset of fibrinolysis was not delayed at hypothermic temperatures, but the rate of lysis was significantly decreased. CORM-2 exposure increased velocity of clot growth and strength, delayed the onset of fibrinolysis, and slowed the rate of lysis.

   Biospecimens

   • Bodily Fluid - Plasma

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Morphology	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen components	DMSO added DMSO and CORM-2 added
   Hematology/ auto analyzer Specific	Incubation temperature	37 degrees C 35 degrees C 33 degrees C 31 degrees C 29 degrees C 27 degrees C

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