Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Influence of the centrifuge time of primary plasma tubes on routine coagulation testing.

Author(s): Lippi G, Salvagno GL, Montagnana M, Manzato F, Guidi GC

Publication: Blood Coagul Fibrinolysis, 2007, Vol. 18, Page 525-8

PubMed ID: 17581331 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sequential collection and centrifugation duration on the measurement of coagulation and hematological analytes. Five blood specimens were collected from 10 fasting volunteers in trisodium citrate tubes after a single venipuncture. Sequentially collected aliquots were centrifuged at room temperature at 1,200 x g for increasing durations (1, 2, 5, 10 or 15 min) such that the 5th aliquot was always centrifuged for 15 min, while the first aliquot was centrifuged for just 1 min.

   Summary of Findings:

   Compared to specimens centrifuged for the recommended 15 min, specimens centrifuged for 10 min or less had increased platelet counts (p<0.01), and those centrifuged for 5 min or less had increased red blood cell counts (p<0.05). In addition to these changes, centrifugation times of 2 min or less led to increased APTT and fibrinogen levels compared to those centrifuged for 15 min. Further, in addition to the above changes, a centrifugation time of 1 min resulted in increased white blood cell counts (p<0.01). Prothrombin time (PT) was unaffected by centrifugation times of less than 15 min. The changes in fibrinogen and aPTT when centrifugation time was decreased to less than 2 and 1 min, respectively, were clinically relevant.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Morphology	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot sequential collection	1st collection 2nd collection 3rd collection 4th collection 5th collection
   Biospecimen Aliquots and Components	Centrifugation	Multiple durations compared

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