Influence of the needle bore size on platelet count and routine coagulation testing.

Author(s): Lippi G, Salvagno GL, Montagnana M, Poli G, Guidi GC

Publication: Blood Coagul Fibrinolysis, 2006, Vol. 17, Page 557-61

PubMed ID: 16988551 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of needle gauge on platelet counts, PT, APTT, and fibrinogen, D-dimer, and free hemoglobin levels in plasma. Three sequential venipunctures, on alternating arms and different veins, were used to collect specimens within 5 min.

   Summary of Findings:

   With specimens collected using a 21-gauge needle used as a reference, D-dimer levels were significantly higher when blood was collected using a 25-gauge needle (p<0.001), and platelet counts were significantly lower when collected using a 23-gauge or 25-gauge needle (both p<0.001). No significant differences were seen between 21-gauge needle specimens and 23-gauge or 25-gauge needle specimens with respect to APTT, PT, fibrinogen, or free hemoglobin.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Peptide	ELISA
   Morphology	Hematology/ auto analyzer
   Glycoprotein	Hematology/ auto analyzer
   Cell count/volume	Hematology/ auto analyzer
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Needle gauge	21-gauge 23-gauge 25-gauge
   Biospecimen Aliquots and Components	Aliquot sequential collection	2nd collection 3rd collection 1st collection

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