Influence of blood sampling on protein profiling and pattern analysis using matrix-assisted laser desorption/ionisation mass spectrometry.
Author(s): Pelzer AE, Feuerstein I, Fuchsberger C, Ongarello S, Bektic J, Schwentner C, Klocker H, Bartsch G, Bonn GK
Publication: BJU Int, 2007, Vol. 99, Page 658-62
PubMed ID: 17407520 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of collection tube type on MALDI-TOF MS analysis and levels of PSA and hormones among healthy volunteers and prostate cancer patients. Serum and plasma were aliquoted and stored at -80 degrees C until analysis.
Summary of Findings:
With the exception of fPSA in specimens from prostate cancer patients, there were no statistically significant differences in tPSA, fPSA, f/tPSA, cPSA, LH, FSH, testosterone, free testosterone, or estrogen levels between specimens collected in Vacuette tubes containing a serum separator gel barrier and those collected in Monovette tubes with no gel barrier from healthy volunteers or individuals with prostate cancer. There were 7 peaks found during MALDI-TOF MS analysis that differentiated between collection tube types among healthy volunteers and 4 peaks that differentiated between collection tube types among prostate cancer patients. All of these peaks, except for one, were also included in the 177 discriminatory peaks between prostate cancer patients and healthy volunteers. The authors concluded that the one peak at 6941 m/z was representative of a peptide produced during specimen collection.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Normal
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform Glycoprotein Immunoassay Steroid Immunoassay Protein MALDI-TOF MS Peptide MALDI-TOF MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Acquisition Type of collection container/solution Vacuette tubes (gel barrier)
Monovette tubes