Isolation of biologically active RNA from human autopsy for the study of cystic fibrosis.

Author(s): Humphreys-Beher MG, King FK, Bunnel B, Brody B

Publication: Biotechnol Appl Biochem, 1986, Vol. 8, Page 392

PubMed ID: 3768147 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this paper was to evaluate the yield and biological integrity of RNA isolated from postmortem lung, spleen, and stomach collected at different postmortem intervals (1.5-21 h).

   Summary of Findings:

   The authors report that non-degraded RNA may be isolated from lung tissue up to 20 hours postmortem, but spleen and stomach must be isolated within 2 hours postmortem and should be promptly immersed in guanidine isothiocyanate or an equivalent method of nuclease inactivation. RNA yields from stomach and spleen were insufficient for further evaluation. Lung specimens for which distinct ribosomal bands were observed yielded sufficient RNA for cDNA library construction and the generation of in vitro translation products.

   Biospecimens

   • Tissue - Lung
   • Tissue - Stomach
   • Tissue - Spleen

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Cystic Fibrosis
   • Autopsy
   • Not specified

   Platform:

   Analyte	Technology Platform
   RNA	Electrophoresis
   RNA	In vitro translation
   RNA	First-strand cDNA Synthesis

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Biospecimen location	Lung Spleen Stomach
   Preaquisition	Postmortem interval	1.5 h 2.0 h 8.0 h 18.0 h 20.0 h

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