Tissue microarray-based immunohistochemical study can significantly underestimate the expression of HER2 and progesterone receptor in ductal carcinoma in situ of the breast.

Author(s): Lin Y, Hatem J, Wang J, Quinn A, Hicks D, Tang P

Publication: Biotech Histochem, 2011, Vol. 86, Page 345-50

PubMed ID: 20701550 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to compare ER, PR, and HER2 IHC staining of TMA with staining seen in whole sections of ductal carcinoma in situ of the breast. Concordance rates between the two types of slides were evaluated with respect to core number on the TMA. The authors defined positive TMA results as staining in at least 1 of 3 cores.

   Summary of Findings:

   The specificity and sensitivity rates of TMA-based ER IHC were 87.0 and 90.4%, respectively when whole sections were used as the reference. For PR and HER2, the specificity and sensitivity rates were 75.9 and 76.1% and 90.6 and 27.3%, respectively. When the number of TMA cores evaluated was increased from 1 to 2 and 3, non-concordance rates (including discordance and non-informative results) between TMA and whole sections decreased from 46.5% to 22.7% and 11.6%, respectively (p<0.001). Tumor size had no effects on non-concordance rates between TMA and whole sections which were 33.3%, 37.1%, and 39.3% for tumor sizes < 1 cM, 1-2 cM, and > 2 cM, respectively.

   Biospecimens

   • Tissue - Breast

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Immunohistochemistry Specific	Targeted peptide/protein	ER PR HER2
   Biospecimen Aliquots and Components	Type of slide	TMA Whole section
   Biospecimen Aliquots and Components	Aliquot size/volume	1 TMA core 2 TMA cores 3 TMA cores < 1 cM tumor size 1-2 cM tumor size > 2 cM tumor size

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