Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Effects of time and temperature during attachment of sections to microscope slides on immunohistochemical detection of antigens.

Author(s): Jones WT, Stockard CR, Grizzle WE

Publication: Biotech Histochem, 2001, Vol. 76, Page 55-8

PubMed ID: 11440305 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of time and temperature during tissue section attachment to slides on IHC staining using a panel of 11 different antibodies and 9 neoplastic and benign tissues including colon and other unspecified tissues. Plus glass microscope slides were used.

   Summary of Findings:

   Attachment times of 1, 4, or 24 h did not affect immunostaining intensity of B-cell lymphoma 2 (Bcl-2), and the authors state that generally, immunostaining intensities of the other tested antigens were also unaffected by this range of slide attachment times. There were no differences in the intensity of proliferating cell nuclear antigen (PCNA) immunostaining with slide attachment temperatures of 58 or 68 degrees C (16 h attachment). However, a slide attachment temperature of 80 degrees C for 16 h tended to decrease immunostaining intensity for PCNA and, the authors state, all other antigens tested.

   Biospecimens

   • Tissue - Colorectal
   • Tissue - Other

   Preservative Types

   • Formalin

   Diagnoses:

   • Neoplastic
   • Neoplastic - Benign

   Platform:

   Analyte	Technology Platform
   Protein	Immunohistochemistry
   Morphology	Light microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Tissue section adhesion	1 h 4 h 16 h 24 h 58 degrees C 68 degrees C 80 degrees C

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