Viable mononuclear cell stability study for implementation in a proficiency testing program: impact of shipment conditions.
Author(s): Kofanova OA, Davis K, Glazer B, De Souza Y, Kessler J, Betsou F
Publication: Biopreserv Biobank, 2014, Vol. 12, Page 206-16
PubMed ID: 24955735 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effect of cryopreservation or storage solution, short-term storage, and method of analysis on the viability of peripheral blood mononuclear cells (PBMC) after shipping.
Conclusion of Paper
Cryopreservation solution had no effect on the viability of liquid nitrogen-shipped, high viability (HV) PBMC. When the cells were considered to have intermediate viability (IV), viability was higher in cells shipped in cryostor 10 than 10% DMSO, but the difference was only significant when assessed by flow cytometer or ELISpot and not by hemocytometer. Ambient temperature shipping and short-term storage for up to 3 days rather than 1 day in AQIX RS-1 had no effect on the viability of HV or IV PBMC. While the viability of HV PBMC was comparable when measured by hemocytometer and flow cytometer, viability of IV PBMC was lower by flow cytometer than hemocytometer among specimens shipped at ambient temperatures.
Studies
-
Study Purpose
The purpose of this study was to determine the effect of cryopreservation or storage solution, short-term storage at room temperature and method of analysis on the viability of PBMC after shipping. PBMC were obtained from an unspecified number of pooled acid-citrate-dextrose (ACD) blood specimens. Specimens were considered HV PBMC immediately after isolation and IV after 1 week at 4°C.
Summary of Findings:
Cryopreservation solution had no effect on the viability of liquid nitrogen-shipped, HV PBMC. When the cells were considered to have IV (stored for 1 week at 4°C), viability was higher in cells shipped in cryostor 10 than 10% DMSO, but the difference was only significant when assessed by flow cytometer or ELISpot and not by hemocytometer. Ambient temperature shipping and short-term storage for 3 days rather than 1 in AQIX RS-1 had no effect on the viability of HV or IV PBMC. While the viability of HV PBMC was comparable when measured by hemocytometer and flow cytometer, viability of IV PBMC was lower by flow cytometer than hemocytometer among specimens shipped at ambient temperature.
Biospecimens
Preservative Types
- None (Fresh)
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Cell count/volume ELISpot Cell count/volume Light microscopy Cell count/volume Flow cytometry Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Between site transportation method Mailed
Storage Storage conditions Ambient temperature
Liquid nitrogen
Storage Short-term storage solution 10% DMSO
Cryostor 10
AQIX RS-1
Storage Storage duration 0 days
1 week
Storage Time at room temperature 1 day
2 days
Flow cytometry Specific Technology platform Hemocytometer