SPIN: Development of Sample-specific Protein Integrity Numbers as an Index of Biospecimen Quality
Author(s): Geddes TJ, Ahmed S, Pruetz BL, Larson DM, Thibodeau BJ, Akervall J, Wilson GD
Publication: Biopreserv Biobank, 2013, Vol. 11, Page 25-32
Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to determine the effects of room temperature storage and freeze-thaw cycling on SELDI-TOF mass spectrometry peak identification and quantification of specific proteins by ELISA in plasma and serum. Plasma and serum were frozen with a thermo conductive freezing system and stored at -80 degrees C.
Summary of Findings:
A total of 445 and 75, peak clusters were observed in plasma and serum, respectively. In plasma, 8 peaks increased with room temperature storage and 1 peak decreased. In serum, 17 peaks were affected by storage at room temperature or freeze-thaw cycling. The mean SPIN determined based on 3 peak clusters that decreased and 1 that was stable was for serum (not calculated for plasma). Serum specimens with weak and strong SPIN numbers had similar levels of c-peptide.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Protein SELDI-TOF MS Protein ELISA Peptide SELDI-TOF MS Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Storage Time at room temperature 0 min
30 min
60 min
120 min
Storage Freeze/thaw cycling 0 cycles
20 cycles
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Study Purpose
The purpose of this study was to determine the effects of freeze-thaw cycling on SELDI-TOF mass spectrometry peak identification and quantification of specific proteins by ELISA in urine. Urine was frozen at -80 degrees C until analysis and subjected to overnight incubation at room temperature for freeze-thaw cycling.
Summary of Findings:
A total of 443 peak clusters were observed in urine. 3 peak clusters were shown to decrease after 20 freeze-thaw cycles in urine. The mean SPIN determined based on 3 peak clusters that decreased and 1 that was stable was 0.39 for urine. Urine specimens with strong SPIN (ideal processing) had higher levels of IL-2 and IL-6 than specimens with weak SPIN (room temperature storage or freeze-thaw cycling).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Not specified
Platform:
Analyte Technology Platform Peptide SELDI-TOF MS Protein SELDI-TOF MS Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Freeze/thaw cycling 0 cycles
20 cycles
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Study Purpose
The purpose of this study was to determine the effects of room temperature storage and freeze-thaw cycling on SELDI-TOF mass spectrometry peak identification and quantification of specific proteins by ELISA in thyroid papillary carcinoma tissue and periprosthetic spinal vertebral tissue specimens. Periprosthetic spinal vertebral tissue was placed into RNAlater, minced and frozen at -80 degrees C. Details of processing of thyroid papillary carcinoma specimens were not specified.
Summary of Findings:
A total of 272 and 338 peak clusters were observed in thyroid papillary carcinoma tissue and periprosthetic spinal vertebral tissue, respectively. 4 peak clusters decreased with room temperature storage of periprosthetic spinal vertebral tissue, while 9 clusters increased and 8 peak clusters decreased with room temperature storage of thyroid papillary carcinoma specimens. The mean SPIN determined based on 3 peak clusters that decreased and 1 that was stable were 0.82 and 0.59 for thyroid papillary carcinoma tissue, and periprosthetic spinal vertebral tissue, respectively. However, thyroid papillary carcinoma tissue, and periprosthetic spinal vertebral tissue specimens with strong SPIN (ideal processing) had higher levels of IL-2 than specimens with weak SPIN (room temperature storage or freeze-thaw cycling).
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Neoplastic - Carcinoma
- Not specified
Platform:
Analyte Technology Platform Peptide SELDI-TOF MS Protein SELDI-TOF MS Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Freeze/thaw cycling 0 cycles
20 cycles
Biospecimen Acquisition Biospecimen location Thyroid
Periprosthetic spine
Storage Time at room temperature 0 min
30 min
60 min
120 min