Biological stability of mRNA isolated from human postmortem brain collections.
Author(s): Leonard S, Logel J, Luthman D, Casanova M, Kirch D, Freedman R
Publication: Biol Psychiatry, 1993, Vol. 33, Page 456
PubMed ID: 8098224 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
-
Study Purpose
The purpose of this study was to evaluate the influence of postmortem interval, on-ice dissection time, and frozen storage duration on the quality and quantity of RNA isolated from postmortem brain tissue.
Summary of Findings:
The authors report that postmortem interval, dissection time delay, and frozen storage time did not influence RNA yield. The amount of degradation detected via Northern blot for beta-actin mRNA increased with the duration of frozen storage but not postmortem interval or dissection time. The authors recommend that postmortem brain specimens used for in vitro translation should be collected within 1 hour of death and subjected to frozen storage for no more than 1 year, although optimal results were obtained with freshly collected specimens. PCR amplification of APRT, bFGF, and FGFr genes in random hexamer primered samples was successful among all specimen handling groups.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Autopsy
Platform:
Analyte Technology Platform RNA Northern blot RNA RT-PCR RNA Spectrophotometry RNA First-strand cDNA Synthesis RNA In vitro translation Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Postmortem interval 7 h
14 h
21 h
30.5 h
40.5 h
42.5 h
Storage Storage duration > 3 months at -70 degrees C
4 - 6 years at -70 degrees C
Storage Storage duration Dissection time delay of 1.25 h
Dissection time delay of 3.75 h
Dissection time delay of 5.00 h
Dissection time delay of 6.00 h