Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Short- and long-term stability of synovial fluid calprotectin.

Author(s): Čičak H, Bulat S, Jeličić J, Ivković A, Maštrović Radončić K, Radišić Biljak V, Dukić L, Šimundić AM

Publication: Biochem Med (Zagreb), 2024, Vol. 34, Page 030704

PubMed ID: 39435164 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study investigated the stability of calprotectin in synovial fluid stored at 4-8°C for up to 7 days and at -20°C and -80°C for up to 6 weeks. Synovial fluid was collected during arthrocentesis from 11 patients with an inflammatory joint disease; specimens were immediately transported to the laboratory at room temperature (transport time not specified). Case-matched specimens were transferred into plastic tubes without additives and hyaluronidase solution was added. Specimens were mixed by inversion 3-5 times, stored at room temperature for 15 min, and centrifuged at 1500 g for 10 min at room temperature. Synovial fluid was then transferred to Eppendorf LoBind tubes and aliquots were stored at 4-8°C for 0, 1, 2, 3, 4, 5, 6 and 7 days and at -20°C and -80°C for 1, 2, 3, 4, 5 and 6 weeks. Prior to analysis, frozen specimens were thawed and homogenized.  Calprotectin was quantified using the Buhlmann fCAL turbo reagent with the Siemens Atellica Solution instrument. The maximal permissible difference was defined as 9.1% based on the reagent manufacturer’s recommendation.

   Summary of Findings:

   Although calprotectin concentration decreased with synovial fluid storage at 4°C or -20°C and increased with storage at -80°C, the changes were not statistically significant. Further, the differences in stored specimens from baseline (day 0) were well below the predefined maximal permissible difference of 9.1% (≤ 3.36% after 1 week at 4°C, ≤ 4.89% after 6 weeks -20°C and ≤ 4.86% after 6 weeks -80°C). The authors also found that the coefficient of variance (CV) for two different internal quality control lots (83 μg/g and 271 μg/g) that were assessed three times a week was low at both concentrations (5.01% and 4.69%, respectively for 83 μg/g and 2.23% and 3.02%, respectively for 271 μg/g).

   Biospecimens

   • Bodily Fluid - Synovial Fluid

   Preservative Types

   • Frozen
   • None (Fresh)

   Diagnoses:

   • Other diagnoses
   • Arthritis

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Storage temperature	4°C -20°C -80°C
   Storage	Storage duration	0 days 1 day 2 days 3 days 4 days 5 days 6 days 7 days 1 week 2 weeks 3 weeks 4 weeks 5 weeks 6 weeks

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