Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Plasma free DNA: Evaluation of temperature-associated storage effects observed for Roche Cell-Free DNA collection tubes.

Author(s): Enko D, Halwachs-Baumann G, Kriegshäuser G

Publication: Biochem Med (Zagreb), 2019, Vol. 29, Page 010904

PubMed ID: 30799979 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study investigated the effects of storing whole blood at room temperature and 4-8˚C in Roche Cell-Free DNA Collection Tubes on cfDNA concentrations in plasma. Whole blood was collected from 30 healthy individuals into five Roche Cell-Free DNA Collection Tubes. Tubes were centrifuged immediately, after 24 or 48 h at room temperature, or after 24 or 48 h at 4-8˚C. After storage, plasma was obtained by centrifugation at 1600 x g for 15 min. cfDNA was extracted from 2 mL of plasma using the QIAamp Circulating Nucleic Acid Kit using a QIAsymphony. cfDNA was stored at -60˚C until analysis. cfDNA was quantified by amplification of a 110 bp fragment of β-globin using EvaGreen-based Droplet Digital PCR.

   Summary of Findings:

   cfDNA levels in plasma were not significantly affected by storage of whole blood in Roche Cell-Free DNA Tubes for 24 or 48 h at either room temperature or 4-8˚C. The authors report visible hemolysis in all specimens stored for 48 h at 4-8˚C.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   DNA	Digital PCR

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated
   Storage	Storage duration	0 h 24 h 48 h
   Storage	Storage temperature	Room temperature 4-8˚C
   Biospecimen Aliquots and Components	Hemolysis	Present Absent

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