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Fasting conditions: Influence of water intake on clinical chemistry analytes.

Author(s): Benozzi SF, Unger G, Campion A, Pennacchiotti GL

Publication: Biochem Med (Zagreb), 2018, Vol. 28, Page 010702

PubMed ID: 29187795 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study compared levels of 16 clinical chemistry analytes in plasma from women before and one hour after consumption of 300 mL water. Blood was collected using 21-gauge needles from twenty healthy women who had refrained from exercise for 24 h before phlebotomy. The first specimen was collected following a 12 h fast without water intake and the second was collected an hour after consuming 300 mL of water. After collection, blood was aliquoted into lithium heparin tubes and centrifuged at 15,000 x G for 15 min at room temperature. Plasma was then placed in tubes containing no additives and analyzed using clinical chemistry analyzers. 

   Summary of Findings:

   Compared to fasting specimens, those obtained 1 h after water consumption had significantly higher total protein (74 g/L versus 73 g/L, P<0.001), urea (4.16 mmol/L versus 4.08 mmol/L, P=0.01), total bilirubin (13 µmol/L versus 12 µmol/L, P=0.021), total cholesterol (4.9 mmol/L versus 4.9 mmol/L, P=0.042), triglycerides (1.06 mmol/L versus 1.05 mmol/L, P=0.002), UA (270 µmol/L versus 260 µmol/L, P=0.006), GGT (12 U/L versus 12 U/L, P=0.046), AST (24 U/L versus 22 U/L, P<0.001), and LD (386 U/L versus 364 U/L, P<0.001). However, they were not considered clinically relevant as none of the changes exceeded the RCV. Levels of glucose, creatinine, cystatin C, high density lipoprotein (HDL) cholesterol, low density lipoprotein (LDL) cholesterol, C-reactive protein, and alanine aminotransferase (ALT) were unaffected by water consumption.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Carbohydrate	Clinical chemistry/auto analyzer
   Lipid	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Steroid	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer
   Lipoprotein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Preaquisition	Diagnosis/ patient condition	1 h after consuming 300 mL water Fasting
   Biospecimen Acquisition	Time of biospecimen collection	Fasting 1 h after consuming 300 mL water

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