Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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U6 is not a suitable endogenous control for the quantification of circulating microRNAs.

Author(s): Xiang M, Zeng Y, Yang R, Xu H, Chen Z, Zhong J, Xie H, Xu Y, Zeng X

Publication: Biochem Biophys Res Commun, 2014, Vol. 454, Page 210-4

PubMed ID: 25450382 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of freeze-thaw cycling and diagnosis on serum levels of U6, miR-16, and miR-24. Serum from 80 cancer patients (20 each of gastric, breast, colorectal and nasopharyngeal cancers) and from 30 healthy volunteers was stored frozen at -80°C. RNA was isolated using the mirVana PARIS kit, and miRNA was quantified using TaqMan low density arrays (TLDA). The number of serum specimens included in freeze-thaw cycling experiments was unspecified.

   Summary of Findings:

   While U6 CT values increased with freeze-thaw cycling of serum, miR-16 and miR-24 CT values remained consistent through 4 freeze-thaw cycles. The expression of U6 was higher in serum from patients with nasopharyngeal carcinoma than in control patients, and when U6 levels were investigated in 30 healthy individuals and 80 cancer patients, a high degree of variability was observed. In contrast, miR-16 levels were very stable.

   Biospecimens

   • Bodily Fluid - Serum

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal
   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   RNA	Low density array

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles 4 cycles
   Preaquisition	Diagnosis/ patient condition	Healthy Nasopharyngeal carcinoma
   Low density array Specific	Targeted nucleic acid	U6 miR-16 miR-24

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