Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

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Hemolysis in needleless connectors for phlebotomy.

Author(s): Sharp MK, Mohammad SF

Publication: ASAIO J, 2003, Vol. 49, Page 128-30

PubMed ID: 12558320 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of needle gauge, the use of needleless connectors, and different collection containers on hemolysis. All blood draws simulated venipuncture by drawing blood from a bottle through the needles or connectors into a vacutainer tube or syringe.

   Summary of Findings:

   The use of needleless connectors resulted in more hemolysis than blood collection using needles of any gauge, but regardless of collection method, hemolysis was low (<43 mg/dL) and unlikely to interfere with clinical assays. Specimens collected using the PosiFlow needless connector had more hemolysis than those collected with the Clave needleless connector (p=0.0008). Of the needles, use of the 18 gauge needle resulted in the most hemolysis and the 22 gauge needle resulted in the least (p=0.008). Using a vacutainer instead of a syringe for blood collection slightly increased hemolysis (p=0.023).

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Needle Needleless connector Vacuum tube Syringe draw
   Biospecimen Acquisition	Needle gauge	18 16 22
   Biospecimen Acquisition	Type of collection container/solution	Syringe Vacutainer tube

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