Increased levels of circulating intercellular adhesion molecule 1 in the sera of patients with rheumatoid arthritis.
Author(s): Cush JJ, Rothlein R, Lindsley HB, Mainolfi EA, Lipsky PE
Publication: Arthritis Rheum, 1993, Vol. 36, Page 1098-102
PubMed ID: 8102057 PubMed Review Paper? No
Suggested by: ISBER
Purpose of Paper
The purpose of this paper was to determine the effects of storage of serum and synovial fluid specimens for 18 h on concentrations of intracellular adhesion molecule 1 (ICAM-1) in normal controls and patients with rheumatoid or other forms of arthritis.
Conclusion of Paper
ICAM-1 levels were not affected by storage of serum or synovial fluid at 37 or 4 degrees C for 18 h. Further, the presence of synovial fluid or recombinant ICAM-1 during storage of serum did not alter ICAM-1 levels. The concentration of ICAM-1 was higher in serum than in synovial fluid in patients with rheumatoid or osteoarthritis. Higher levels of ICAM-1 were found in serum from patients with rheumatoid arthritis than were found in serum or synovial fluid specimens from patients with osteoarthritis, inflammatory arthritis or in normal controls. Serum ICAM-1 levels correlated with joint score and erythrocyte sedimentation rate (ESR).
Studies
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Study Purpose
The purpose of this study was to determine the effects of storage at 4 or 37 degrees C for 18 h on concentrations of ICAM-1 in serum and synovial fluid specimens from normal controls and patients with rheumatoid or other forms of arthritis.
Summary of Findings:
ICAM-1 levels were not affected by storage of serum or synovial fluid at 37 or 4 degrees C for 18 h. Further, the presence of synovial fluid or recombinant ICAM-1 during storage of serum did not alter ICAM-1 levels. The concentration of ICAM-1 was higher in serum than in synovial fluid in patients with rheumatoid or osteoarthritis. Higher levels of ICAM-1 were found in serum from patients with rheumatoid arthritis than were found in serum or synovial fluid specimens from patients with osteoarthritis, inflammatory arthritis or in normal controls. Serum ICAM-1 levels correlated with joint score and ESR.
Biospecimens
Preservative Types
- Frozen
Diagnoses:
- Arthritis
- Osteoarthritis
- Rheumatoid Arthritis
- Normal
- Lupus
Platform:
Analyte Technology Platform Protein ELISA Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Preaquisition Diagnosis/ patient condition Rheumatoid arthritis
Osteoarthritis
Inflammatory arthritis
Normal control
Storage Storage temperature 37 degrees C
4 degrees C
Storage Storage duration 18 h
Storage Storage conditions With synovial fluid
Without synovial fluid
Biospecimen Acquisition Biospecimen location Synovial fluid
Serum