A new study of intraosseous blood for laboratory analysis.

Author(s): Miller LJ, Philbeck TE, Montez D, Spadaccini CJ

Publication: Arch Pathol Lab Med, 2010, Vol. 134, Page 1253-60

PubMed ID: 20807043 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of using IO verses peripheral venous blood for CBC analysis and the measurement of 10 common clinical chemistry analytes. Specimens from 9 volunteers were included in the analysis. Specimens intended for clinical chemistry analysis were centrifuged, and serum was transferred to new tubes. Hematology specimens were transferred to potassium EDTA tubes. All specimens were kept at ambient temperatures while transported to the laboratory where analysis took place within 1-6 hours of blood collection.

   Summary of Findings:

   There were significant correlations between values obtained from IO blood and peripheral venous blood for red blood cell counts, hemoglobin, hematocrit, glucose, blood urea nitrogen, creatinine, chloride, total protein, and albumin. However, no correlations between values obtained from the two types of specimens were seen for white blood cell or platelet counts, sodium, potassium, carbon dioxide, or calcium concentrations. IO sodium, calcium, creatinine, potassium, and carbon dioxide values were within 5, 10, 25, 25, and 25% of average peripheral venous values, respectively. The first and second 4 mL aliquots of IO blood, taken after discarding 2 mL or 6 mL, respectively, had significantly correlated values for red blood cell counts, hemoglobin, hematocrit, glucose, blood urea nitrogen, creatinine, chloride, total protein, carbon dioxide, and albumin. However, white blood cell and platelet counts, sodium, potassium, and calcium concentrations were not significantly correlated between the first and 2nd aliquots of IO blood.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer
   Protein	Hematology/ auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Protein	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Aliquot sequential collection	1st collection 2nd collection
   Biospecimen Aliquots and Components	Aliquot size/volume	4 mL (IO) 10-12 mL (venipuncture)
   Biospecimen Acquisition	Anatomical location of blood draw	Intraosseous collection Peripheral vein

   View more