Methodological and preanalytical evaluation of a RAGE immunoassay.

Author(s): Wittwer C, Lehner J, Fersching D, Siegele B, Stoetzer OJ, Holdenrieder S

Publication: Anticancer Res, 2012, Vol. 32, Page 2075-8

PubMed ID: 22593491 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of collecting plasma rather than serum, delayed centrifugation, storage, and freeze-thaw cycling on serum sRAGE levels.

   Summary of Findings:

   EDTA-plasma, heparinated-plasma and citrated-plasma had 95.4%, 97% and 88.1% as much sRAGE as serum. There was no effect of storing serum for up to 7 days at 4 or 25 degrees C or of a 6 h or 24 h room temperature delay before centrifugation. Further, up to 3 freeze-thaw cycles of serum did not significantly affect sRAGE levels compared to serum thawed once. The intra-assay imprecision of measurement of sRAGE in serum was 5.6-11%.

   Biospecimens

   • Bodily Fluid - Plasma
   • Bodily Fluid - Serum
   • Bodily Fluid - Blood

   Preservative Types

   • Frozen

   Diagnoses:

   • Normal

   Platform:

   Analyte	Technology Platform
   Protein	ELISA

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Freeze/thaw cycling	1 cycle 2 cycles 3 cycles
   Storage	Storage temperature	4 degrees C 25 degrees C
   Storage	Storage duration	0 h 6 h 24 h 48 h 7 days
   Storage	Time at room temperature	0 h 6 h 24 h
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Acquisition	Anticoagulant	Citrate EDTA Heparin None
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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