Cancer Diagnosis Program | Biorepositories and Biospecimen Research Branch

Search BRD Papers

Circulating tumor cells in metastatic breast cancer: timing of blood extraction for analysis.

Author(s): Martín M, García-Sáenz JA, Maestro De las Casas ML, Vidaurreta M, Puente J, Veganzones S, Rodríguez-Lajusticia L, De la Orden V, Oliva B, De la Torre JC, López-Tarruella S, Casado A, Sastre J, Díaz-Rubio E

Publication: Anticancer Res, 2009, Vol. 29, Page 4185-7

PubMed ID: 19846970 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   This study explored how circadian rhythm may influence the number of circulating tumor cells (CTCs) detected by the CellSearch method in peripheral blood of metastatic breast cancer patients. Blood (7.5 mL) was collected in CellSave tubes at 8:00 a.m. and 8:00 p.m. of the same day from 51 patients with metastatic breast cancer and processed using the CellSearch method within 72 h. Control specimens were collected at 8:00 a.m. from healthy, age-matched volunteers and run in parallel.

   Summary of Findings:

   No significant differences in numbers of CTCs in metastatic breast cancer patient specimens were observed between case-matched peripheral blood specimens collected 12 hours apart (P=0.8427). Prognostic classification was unchanged in blood specimens collected 12 hours apart in 96% of patients when a threshold of ≥5 CTCs/7.5mL was applied, as only two of the 51 patients were reclassified based on the time of CTC collection (patient 1: 0 vs. 10 CTCs; patient 2: 8 vs. 3 CTCs). No CTCs were isolated from healthy control specimens.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • Other Preservative

   Diagnoses:

   • Neoplastic - Carcinoma

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Fluorescent microscopy

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Time of biospecimen collection	8:00 a.m. 8:00 p.m.

   View more