Higher amount of free circulating DNA in serum than in plasma is not mainly caused by contaminated extraneous DNA during separation.
Author(s): Umetani N, Hiramatsu S, Hoon DS
Publication: Ann N Y Acad Sci, 2006, Vol. 1075, Page 299-307
PubMed ID: 17108224 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
Studies
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Study Purpose
The purpose of this study was to compare the amount of circulating cell-free DNA in serum and plasma from cancer patients and those with benign tumors. Specimens were drawn into either CORVAC serum separator tubes or sodium citrate tubes and processed within 6 hours of blood draw. Serum and plasma were filtered and used directly as templates for real time qPCR of ALU repeats.
Summary of Findings:
The average concentration of DNA was 970 pg/uL in serum and 180 pg/uL in plasma. The amount of DNA in serum was significantly higher than that in plasma (p<0.0001), but the two values were significantly and strongly correlated (r=0.72, p=0.0002).
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Neoplastic - Benign
- Neoplastic - Carcinoma
Platform:
Analyte Technology Platform DNA Real-time qPCR Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Real-time qPCR Specific Targeted nucleic acid ALU repeats
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum