The effect of recentrifugation of serum separator tubes on concentration of serum analytes.
Author(s): Shafi H, Sadrzadeh H
Publication: Ann Clin Lab Sci, 2012, Vol. 42, Page 318-9
PubMed ID: 22964622 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of recentrifugation of serum separator tubes (SST) following room temperature or refrigerated storage on levels of 10 clinical chemistry analytes.
Conclusion of Paper
When specimens were stored in the SST and recentrifuged prior to analysis, significantly higher levels of glucose, sodium, potassium creatinine, bicarbonate, phosphorus, and magnesium were measured than when analyzed immediately following the initial centrifugation.
Studies
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Study Purpose
The purpose of this study was to determine the effects of recentrifugation of SST following room temperature or refrigerated storage on levels of 10 clinical chemistry analytes. Blood was collected from 6 healthy individuals in SST. All tubes were centrifuged and assayed prior to room temperature or refrigerated storage and then again after storage. This study does not separately analyze the effects of post-centrifugation storage and a second centrifugation step.
Summary of Findings:
After storage at room temperature for 2-24 h and recentrifugation, levels of glucose (p=0.003), sodium (p=0.005), creatinine (p<0.0001), bicarbonate (p=0.004), phosphorus (p=0.01), and magnesium (p=0.002) were higher than when assayed immediately after the initial centrifugation. Compared to baseline, specimens stored at 4°C for 2-24 h and recentrifuged had higher levels of potassium (p=0.004), creatinine (p<0.0001), bicarbonate (p=0.001), phosphorus (p=0.004), and magnesium (p=0.04) than when assayed immediately after the initial centrifugation. Blood urea nitrogen (BUN), chloride, and calcium were not affected by storage (either temperature) and recentrifugation.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Carbohydrate Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Different number of centrifugation steps compared
Storage Storage temperature 4°C
Room temperature
Storage Storage duration 0 h
2 h
4 h
8 h
24 h