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Evaluation of the i-STAT Portable Clinical Analyzer for point-of-care blood testing in the intensive care units of a university children's hospital.

Author(s): Papadea C, Foster J, Grant S, Ballard SA, Cate JC 4th, Southgate WM, Purohit DM

Publication: Ann Clin Lab Sci, 2002, Vol. 32, Page 231-43

PubMed ID: 12175085 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of analyzer type on clinical chemistry and hematology analysis of pediatric blood specimens.

   Summary of Findings:

   The day to day coefficient of variance (CV) using iSTAT for electrolytes, glucose, urea nitrogen and pH was less than 2% and the CV for oxygen and carbon dioxide were less than 6.5%, but the hematocrit and creatinine were highly variable with CVs of 21 and 23.6%, respectively. The CVs using iSTAT were comparable to the CVs using the VITROS or ABL analyzers for all analytes except creatinine and hematocrit which had CVs of <3% using the VITROS analyzer and <9.1% using the ABL analyzer, respectively. The different cartridges performed similarly for all of the analytes that were analyzed by multiple cartridge types. However, a follow-up study of creatinine levels found less correlation between VITROS values using a new lot of cartridges, resulting in possible clinical differences. In specimens analyzed using iSTAT, a small proportional negative bias was found for ionized calcium (iCa) and partial pressure oxygen (PO2), and a there was a positive bias for creatinine. The iSTAT analyzer was found to have good reproducibility for children under 3 months old and those over 3 months old. The authors conclude that iSTAT is suitable for clinical chemistry and hematology analysis for all analytes except creatinine.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Gas	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Small molecule	pH
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Clinical chemistry/auto analyzer Specific	Technology platform	iSTAT portable clinical analyzer Radiometer ABL 725 blood gas analyzer Vitros 750 chemistry analyzer Coulter STKS hematology analyzer
   Hematology/ auto analyzer Specific	Technology platform	iSTAT portable clinical analyzer Coulter STKS hematology analyzer
   Clinical chemistry/auto analyzer Specific	Detection method	EC8+ cartridge EC6+ cartridge G3+ cartridge Creatinine cartridge
   Preaquisition	Patient age	< 3 months > 3 months

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