Evaluation of a reduced centrifugation time and higher centrifugal force on various general chemistry and immunochemistry analytes in plasma and serum.
Author(s): Møller MF, Søndergaard TR, Kristensen HT, Münster AB
Publication: Ann Clin Biochem, 2017, Vol. 54, Page 593-600
PubMed ID: 27687083 PubMed Review Paper? No
Purpose of Paper
This paper investigated the effects of centrifuging blood for 5 min at 3000 x g rather than the standard of 10 min at 2200 x g on levels of nine clinical chemistry analytes in lithium heparin plasma and serum.
Conclusion of Paper
With the exception of lactate dehydrogenase (LDH) in lithium heparin plasma, the mean bias and coefficient of variance (CV) of all analytes remained within the allowable bias after centrifugation at 3000 x g for 5 min rather than the standard protocol. For LDH, the faster and shorter centrifugation resulted in an increase in the coefficient of variance. In specimens from two patients, the faster and shorter centrifugation resulted in a higher than acceptable bias in protein and free triiodothyronine (FT3) levels. Additionally, two plasma specimens had higher than acceptable hemolysis index after the faster and shorter centrifugation and one serum specimen had higher than acceptable hemolysis index using the standard centrifugation.
Studies
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Study Purpose
This study investigated the effects of centrifuging blood for 5 min at 3000 x g rather than the standard protocol of 10 min at 2200 x g on levels of nine clinical chemistry analytes in lithium heparin plasma. Blood specimens from 40 patients and healthy volunteers were collected in quadruplicate into lithium heparin vacutainer tubes. Within 1 h of venipuncture, two specimens from each patient were centrifuged at 21˚C for 5 min at 3000 x g while the remaining two were centrifuged using the standard protocol of 10 min at 2200 x g. The same acceleration and braking speeds (step 9, 25 s) were used in conjunction with both centrifugation protocols. Levels of calcium (Ca), immunoglobulin M (IgM), phosphorus (P), potassium (K), hemolysis index (Hem), icterus index (ICT), lipaemia index (LIP), and LDH were determined using an Abbott Architect c16000 and levels of cobalamin (vitamin B12), folic acid (FOL), and FT3 were determined using an Abbott Architect i2000SR.
Summary of Findings:
Lithium heparin plasma obtained by centrifugation at 3000 x g for 5 min rather than the standard protocol had higher average levels and an increase in the coefficient of variance of LDH. The average bias in LDH from the alternate protocol was 6.3%, which exceeded the goal of 4.3%. The mean bias and CV of all other analytes remained within the allowable bias after centrifugation at 3000 x g for 5 min rather than the standard protocol. However, the difference in FT3 levels were higher than acceptable in specimens from two patients and the hemolysis index was greater than acceptable for two other patients after centrifugation at 3000 x g for 5 min.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
- Not specified
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Peptide Chemiluminescence immunoassay Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Multiple durations compared
Multiple speeds compared
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Study Purpose
This study investigated the effects of centrifuging blood for 5 min at 3000 x g rather than using the standard protocol (2200 x g for 10 min) on levels of nine clinical chemistry analytes in serum. Blood specimens from 40 patients and healthy volunteers were collected in quadruplicate into serum vacutainer tubes. Specimens were allowed to clot for 30-60 min and then two specimens from each patient were centrifuged at 21˚C for 5 min at 3000 x g while the remaining two were centrifuged at 21˚C for 10 min at 2200 x g (standard protocol). The same acceleration and braking speeds (step 9, 25 s) were used in conjunction with both centrifugation protocols. Levels of Ca, IgM, P, K, hemolysis index, ICT, LIP, and total protein were determined using an Abbott Architect c16000 and levels of vitamin B12, FOL, and FT3 were determined using an Abbott Architect i2000SR.
Summary of Findings:
The mean bias and CV of all analytes remained within the allowable bias after centrifugation at 3000 x g for 5 min rather than the standard protocol. However, the difference in total protein levels was higher than acceptable in specimens from two patients after centrifugation at 3000 x g for 5 min. One specimen centrifuged using the standard protocol had a hemolysis index greater than acceptable.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
- Not specified
Platform:
Analyte Technology Platform Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Clinical chemistry/auto analyzer Small molecule Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Peptide Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Centrifugation Multiple durations compared
Multiple speeds compared