Evaluation of haemoglobin A1c measurement by an enzymatic method using an automated analyser that has an on-board haemolysis system.

Author(s): Matsumoto H, Uchino M, Kato M

Publication: Ann Clin Biochem, 2013, Vol. 50, Page 443-9

PubMed ID: 23897101 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of sampling location within the erythrocyte pack and measurement method on levels of HbA1C in blood from inpatients, outpatients and hospital employees.

   Summary of Findings:

   Regardless of sampling location relative to the bottom of the tube, measured levels of HbA1C were comparable when by the enzymatic assay based BM6010/C and the HPLC based Tosoh G7. The authors report no effect of exogenous acetylated Hb, carbamylated Hb and labile HbA1c on measurement of HbA1c. Levels of HbA1c measured by the BM6010/C were very strongly correlated with those measured by the Primus HPLC (r2=0.997) and Tosoh G7 HPLC (r=0.994), but a bias of 0.6-3.3% was observed using the BM6010/C.

   Biospecimens

   • Cell - Red Blood Cells

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Normal
   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Protein	HPLC

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Biospecimen components	1 mm from bottom 3 mm from bottom 5 mm from bottom 0-500 mg/L acetylated Hb added 0-500 mg/L carbamylated Hb added 0-10 g/L labile HbA1c added
   Clinical chemistry/auto analyzer Specific	Technology platform	Tosoh G7 HPLC BM6010/C Primus HPLC
   Biospecimen Aliquots and Components	Blood and blood products	Multiple sampling positions examined

   View more