The analytic impact of a reduced centrifugation step on chemistry and immunochemistry assays: an evaluation of the Modular Pre-Analytics.

Author(s): Koenders MM, van Hurne ME, Glasmacher-Van Zijl M, van der Linde G, Westerhuis BW

Publication: Ann Clin Biochem, 2012, Vol. 49, Page 468-74

PubMed ID: 22859726 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of centrifuging plasma or serum separately for 10 min at 1885 x g instead of in the analyzer for 5 min at 1885 x g on the levels of 22 clinical chemistry analytes. After centrifugation, all specimens were immediately placed into the analyzer for automated processing.

   Summary of Findings:

   Use of the analyzer's 5 min centrifugation rather than a separate 10 min centrifugation before loading the specimens in the analyzer resulted in no statistical differences and very strong correlations in the levels of all analytes except the hemolysis index in plasma and serum and the lipemia index in plasma. The authors attribute the higher lipemia index after the 5 min centrifugation compared to the 10 min centrifugation (p<0.0001) to turbidity. The authors excluded the hemolysis index from correlation analysis because of too many values below the detection limit of the assay.

   Biospecimens

   • Bodily Fluid - Serum
   • Bodily Fluid - Plasma

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Protein	Clinical chemistry/auto analyzer
   Small molecule	Clinical chemistry/auto analyzer
   Electrolyte/Metal	Clinical chemistry/auto analyzer
   Peptide	Clinical chemistry/auto analyzer
   Carbohydrate	Clinical chemistry/auto analyzer
   Glycoprotein	Clinical chemistry/auto analyzer
   Cell count/volume	Spectrophotometry

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Aliquots and Components	Blood and blood products	Plasma Serum
   Biospecimen Aliquots and Components	Blood processing method	Centrifuged in analyzer Centrifuged manually
   Biospecimen Aliquots and Components	Centrifugation	Automated Manual Multiple durations compared

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