Centrifugation protocols: tests to determine optimal lithium heparin and citrate plasma sample quality.
Author(s): Dimeski G, Solano C, Petroff MK, Hynd M
Publication: Ann Clin Biochem, 2011, Vol. 48, Page 218-22
PubMed ID: 21398434 PubMed Review Paper? No
Purpose of Paper
The purpose of this paper was to determine the effects of centrifugation speed and duration and post-centrifugation storage on the measurement of potassium, lactate dehydrogenase (LD), glucose, and phosphorous in lithium heparin plasma and serum. In addition, the effects of centrifugation speed and duration were determined on prothrombin time (PT), activated partial thromboplastin time (APTT), derived fibrinogen and surface-activated clotting time (SACT) in citrated plasma.
Conclusion of Paper
Post-centrifugation storage of lithium heparin plasma for 6 days at 2-8°C led to increased potassium, LD, and phosphorous and decreased glucose, but storage of lithium heparin plasma for 6 h at 21°C only led to significantly increased LD. Storage of serum for 6 days at 2-8°C led to increases in phosphorous, but no other analytes were affected by post-centrifugation storage of serum, and increases in phosphorous were smaller than those observed after storage of lithium heparin plasma.
Centrifugation of citrate tubes for 3 min at 4000 g produced plasma with an elevated platelet count and significantly lower SACT than that observed after centrifugation for 10 min at 3000 g, but no other analytes were affected. The authors state that lithium heparin plasma quality increased with increasing centrifugation duration and speed, but the differences were less than the standard deviations.
Studies
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Study Purpose
The purpose of this study was to determine the effects of post-centrifugation storage on the measurement of potassium, LD, glucose, and phosphorous in lithium heparin plasma and serum. Blood was obtained from 14 healthy individuals.
Summary of Findings:
Post-centrifugation storage of lithium heparin plasma for 6 days at 2-8°C led to increased potassium, LD, and phosphorous and decreased glucose, but storage of lithium heparin plasma for 6 h at 21°C only led to significantly increased LD. Storage of serum for 6 days at 2-8°C led to increases in phosphorous, but no other analytes were affected by post-centrifugation storage of serum, and increases in phosphorous were smaller than those observed after storage of lithium heparin plasma.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Protein Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Storage Storage duration 0 h
6 h
6 days
Biospecimen Aliquots and Components Blood and blood products Plasma
Serum
Storage Storage temperature 21°C
2-8°C
-
Study Purpose
The purpose of this study was to determine the effects of centrifugation speed and duration on potassium, LD, glucose and phosphorous in lithium heparin plasma and serum and PT, APTT, derived fibrinogen and SACT in citrated plasma. Blood was obtained from 14 healthy individuals. Lithium heparin and citrate tubes were centrifuged within 30 min for 3 min at 4000 g (no temperature specified), 10 min at 3100 g (no temperature specified), or 10 min at 3000 g and 20°C, and lithium heparin tubes were also centrifuged for 4 min at 6000 g and 20°C. Serum specimens were centrifuged for 10 min at 3000 g and 20°C.
Summary of Findings:
Centrifugation of citrate tubes for 3 min at 4000 g produced plasma with an elevated platelet count and significantly lower SACT than after centrifugation for 10 min at 3000 g. Further, with increasing platelet count, the SACT decreased, but no other analytes were affected. The authors state that lithium heparin plasma quality increased with increasing centrifugation duration and speed, but the differences in the values for potassium, glucose, and phosphorous between centrifugation conditions were ≤ 0.1 mmol/L (standard deviations of 0.2-0.3, 0.9-1.0 and 0.13-0.14 mmol/L, respectively), and the differences in LD were limited to ≤10 units/L which was much less than the standard deviation of 28-30 units/L.
Biospecimens
Preservative Types
- None (Fresh)
Diagnoses:
- Normal
Platform:
Analyte Technology Platform Carbohydrate Clinical chemistry/auto analyzer Electrolyte/Metal Clinical chemistry/auto analyzer Glycoprotein Hematology/ auto analyzer Morphology Hematology/ auto analyzer Cell count/volume Hematology/ auto analyzer Protein Clinical chemistry/auto analyzer Pre-analytical Factors:
Classification Pre-analytical Factor Value(s) Biospecimen Aliquots and Components Blood and blood products Plasma
Platelet-poor plasma
Platelet-rich plasma
Biospecimen Aliquots and Components Centrifugation Multiple durations compared
Multiple speeds compared