A prospective study of causes of haemolysis during venepuncture: tourniquet time should be kept to a minimum.

Author(s): Saleem S, Mani V, Chadwick MA, Creanor S, Ayling RM

Publication: Ann Clin Biochem, 2009, Vol. 46, Page 244-6

PubMed ID: 19389888 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of blood sampling method, tourniquet time and multiple venepuncture attempts on haemolysis of blood.

   Summary of Findings:

   Of 353 blood specimens sampled 6.5% showed haemolysis. Staff group performing the blood draw, collection method, tourniquet time and number of attempts at venepuncture each appeared to be univariatly associated with haemolysis. However, after logistic regression analysis, only tourniquet time of greater than 1 minute was significantly associated with increased haemolysis with an odds ratio (OR) of 19.5. Specimen transport method had no effect on hemolysis.

   Biospecimens

   • Bodily Fluid - Blood

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified

   Platform:

   Analyte	Technology Platform
   Cell count/volume	Hematology/ auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Biospecimen Acquisition	Method of fluid acquisition	Tourniquet times compared Vacuum tube Butterfly device Syringe draw Cannula Needle Staff group performing phlebotomy compared
   Biospecimen Aliquots and Components	Aliquot sequential collection	First venipuncture attempt Multiple venipuncture attempts
   Storage	Within hospital transportation method	Hand-delivered Pneumatic tube system

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