Stability of creatinine with delayed separation of whole blood and implications for eGFR.

Author(s): Shepherd J, Warner MH, Kilpatrick ES

Publication: Ann Clin Biochem, 2007, Vol. 44, Page 384-7

PubMed ID: 17594786 PubMed Review Paper? No

Purpose of Paper

The purpose of this paper was to determine the effects of delayed centrifugation and method of analysis on creatinine measurement.

Conclusion of Paper

When centrifugation was delayed by more than 14 h, there was an increase in creatinine levels as measured using the three kinetic Jaffe assays, but creatinine levels were stable after centrifugation delays of up to 31 h when assayed using the two enzymatic assays. The increase in creatinine levels after an 11-28 h delay to centrifugation resulted in decreased estimated glomerular filtration rate (eGFR) which led to a misclassification of chronic kidney disease in 4 of 21 patients.

Studies

  1. Study Purpose

    The purpose of this study was to determine the effects of delayed centrifugation and method of analysis on creatinine measurements in healthy individuals. Blood from 5 individuals was subjected to different delays in centrifugation and analyzed on the 5 different analyzers. Subsequently blood specimens from 24 patients were analyzed within 1 h and after a delay of up to 28 h using the Beckman DXC 800 CRE 3 kinetic Jaffe assay.

    Summary of Findings:

    When centrifugation was delayed by more than 14 h, there was an increase in creatinine levels as measured using the three kinetic Jaffe assays, but creatinine levels were stable after centrifugations delays of up to 31 h when assayed using the two enzymatic assays. The largest increase in measured creatinine concentration after delayed centrifugation occurred with the Beckman DXC 800 CRE 3 kinetic Jaffe assay. The increase in creatinine levels after an 11-28 h delay to centrifugation resulted in decreased eGFR which led to a misclassification of chronic kidney disease in 4 of 21 patients.

    Biospecimens
    Preservative Types
    • None (Fresh)
    Diagnoses:
    • Not specified
    • Other diagnoses
    • Normal
    Platform:
    AnalyteTechnology Platform
    Small molecule Clinical chemistry/auto analyzer
    Pre-analytical Factors:
    ClassificationPre-analytical FactorValue(s)
    Storage Time at room temperature 15 min
    <1 h
    4 h
    8 h
    14 h
    24 h
    31 h
    11-28 h
    Clinical chemistry/auto analyzer Specific Technology platform Beckman DXC 800 CRE 3 kinetic Jaffe
    Bayer Advia kinetic Jaffe
    Roche Modular P-800 kinetic Jaffe
    Roche Modular P-800 creatinine plus enzymatic assay
    Vitros 5.1 enzymatic creatinine assay
    Preaquisition Diagnosis/ patient condition Chronic kidney disease
    Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated

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