Stability of creatinine with delayed separation of whole blood and implications for eGFR.
Author(s): Shepherd J, Warner MH, Kilpatrick ES
Publication: Ann Clin Biochem, 2007, Vol. 44, Page 384-7
PubMed ID: 17594786 PubMed Review Paper? No
Purpose of Paper
Conclusion of Paper
The purpose of this study was to determine the effects of delayed centrifugation and method of analysis on creatinine measurements in healthy individuals. Blood from 5 individuals was subjected to different delays in centrifugation and analyzed on the 5 different analyzers. Subsequently blood specimens from 24 patients were analyzed within 1 h and after a delay of up to 28 h using the Beckman DXC 800 CRE 3 kinetic Jaffe assay.
Summary of Findings:
When centrifugation was delayed by more than 14 h, there was an increase in creatinine levels as measured using the three kinetic Jaffe assays, but creatinine levels were stable after centrifugations delays of up to 31 h when assayed using the two enzymatic assays. The largest increase in measured creatinine concentration after delayed centrifugation occurred with the Beckman DXC 800 CRE 3 kinetic Jaffe assay. The increase in creatinine levels after an 11-28 h delay to centrifugation resulted in decreased eGFR which led to a misclassification of chronic kidney disease in 4 of 21 patients.
- None (Fresh)
- Not specified
- Other diagnoses
Analyte Technology Platform Small molecule Clinical chemistry/auto analyzer
Classification Pre-analytical Factor Value(s) Storage Time at room temperature 15 min
Clinical chemistry/auto analyzer Specific Technology platform Beckman DXC 800 CRE 3 kinetic Jaffe
Bayer Advia kinetic Jaffe
Roche Modular P-800 kinetic Jaffe
Roche Modular P-800 creatinine plus enzymatic assay
Vitros 5.1 enzymatic creatinine assay
Preaquisition Diagnosis/ patient condition Chronic kidney disease
Biospecimen Aliquots and Components Centrifugation Centrifugation delays investigated