Stability of creatinine with delayed separation of whole blood and implications for eGFR.

Author(s): Shepherd J, Warner MH, Kilpatrick ES

Publication: Ann Clin Biochem, 2007, Vol. 44, Page 384-7

PubMed ID: 17594786 PubMed Review Paper? No

Purpose of Paper

Conclusion of Paper

Studies

1. Study Purpose

   The purpose of this study was to determine the effects of delayed centrifugation and method of analysis on creatinine measurements in healthy individuals. Blood from 5 individuals was subjected to different delays in centrifugation and analyzed on the 5 different analyzers. Subsequently blood specimens from 24 patients were analyzed within 1 h and after a delay of up to 28 h using the Beckman DXC 800 CRE 3 kinetic Jaffe assay.

   Summary of Findings:

   When centrifugation was delayed by more than 14 h, there was an increase in creatinine levels as measured using the three kinetic Jaffe assays, but creatinine levels were stable after centrifugations delays of up to 31 h when assayed using the two enzymatic assays. The largest increase in measured creatinine concentration after delayed centrifugation occurred with the Beckman DXC 800 CRE 3 kinetic Jaffe assay. The increase in creatinine levels after an 11-28 h delay to centrifugation resulted in decreased eGFR which led to a misclassification of chronic kidney disease in 4 of 21 patients.

   Biospecimens

   • Bodily Fluid - Blood
   • Bodily Fluid - Serum

   Preservative Types

   • None (Fresh)

   Diagnoses:

   • Not specified
   • Other diagnoses
   • Normal

   Platform:

   Analyte	Technology Platform
   Small molecule	Clinical chemistry/auto analyzer

   Pre-analytical Factors:

   Classification	Pre-analytical Factor	Value(s)
   Storage	Time at room temperature	15 min <1 h 4 h 8 h 14 h 24 h 31 h 11-28 h
   Clinical chemistry/auto analyzer Specific	Technology platform	Beckman DXC 800 CRE 3 kinetic Jaffe Bayer Advia kinetic Jaffe Roche Modular P-800 kinetic Jaffe Roche Modular P-800 creatinine plus enzymatic assay Vitros 5.1 enzymatic creatinine assay
   Preaquisition	Diagnosis/ patient condition	Chronic kidney disease
   Biospecimen Aliquots and Components	Centrifugation	Centrifugation delays investigated

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